Phosphate Buffered Saline (PBS)

Author

Affiliations

Pranav Kumar Mishra

Dept of Surgery, Rush University

Dept of Orthopedic Surgery, Rush University

Overview

Similar pH, electrolytes, and osmolarity to the human body.

• Concentration: 1x
• pH 7.4

Uses

• Washing tissues, cells
• Common base solvent in many solutions

Components

1. \(NaCl\)
2. \(KCl\)
3. \(Na_2HPO_4\)
4. \(KH_2PO_4\)
5. \(diH_20\)
6. 1L sterile container

Preparation for 1L of 1x PBS

1. Add 800 mL of \(diH_20\) a sterile container
2. Add electrolytes
   1. 8g \(NaCl\)
   2. 0.2g \(KCl\)
   3. 1.44g \(Na_2HPO_4\)
   4. 0.245g \(KH_2PO_4\)
3. Adjust pH to 7.4 by titrating appropriate amounts of \(Na_2HPO_4\) or \(KH_2PO_4\)

Preparation for 1L of 10x PBS

1. Add 800 mL of \(diH_20\) a sterile container
2. Add electrolytes
   1. 80g \(NaCl\)
   2. 2g \(KCl\)
   3. 14.4g \(Na_2HPO_4\)
   4. 2.45g \(KH_2PO_4\)
3. Adjust pH to 7.4 by titrating appropriate amounts of \(Na_2HPO_4\) or \(KH_2PO_4\)

References:

1. Phosphate-buffered saline (Pbs). Cold Spring Harb Protoc. 2006;2006(1):pdb.rec8247. doi:10.1101/pdb.rec8247