Adapted from the Cell Press STAR Methods to help produce Structured, Transparent, Accessible Reporting in research.
This page is a living document and a work-in-progress. With the goal of improving research reproducibility, several major journals now require a detailed listing of the key resources required to conduct the experiments described.
Schedule permitting, I will update the “csv databases”, which can be found on GitHub. Using Quarto and Python, the csv files are rendered into HTML and GitHub-flavored Markdown. The markdown file is additionally rendered in my Wordpress post.
---------------------------------------------------------------------------KeyError Traceback (most recent call last)
CellIn[1], line 23 20 os.environ['is_docker'] = 'False' 21 os.environ['repo_root'] = "/home/pranav/work/pranavmishra90/research-reference"---> 23 os.chdir(os.environ['repo_root'])
File <frozen os>:709, in _Environ.__getitem__(self, key) 706 value = self._data[self.encodekey(key)]
707exceptKeyError:
708# raise KeyError with the original key value--> 709raiseKeyError(key) fromNone 710returnself.decodevalue(value)
KeyError: 'repo_root'
Antibodies
<>:12: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
<>:12: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
/tmp/ipykernel_2776/516824930.py:12: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
antibodies = antibodies.astype(str).replace('\.0', '', regex=True)
Acknowledgement: We would like to acknowledge Dr. Thomas Schmid at Rush University for preparing and sharing the Lubricin (PRG4) antibody with our group.
Biological Samples
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Chemicals; peptides; and recombinant proteins
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Experiment
Alcohol 100%
Sigma-Aldrich
65347-M
Histology
EM-400 Embedding Medium Paraffin
Leica
3801320
Histology
HistoChoice Mounting Media
VWR
H157-475ML
Histology
Hydrochloric acid
Sigma-Aldrich
258148-500ML
Histology
Paraformaldehyde powder 95%
Sigma-Aldrich
158127-500G
Histology
Paraplast X-tra
Leica
39603002
Histology
Permount Mounting Medium
Fisher
SP15-500
Histology
Reagent Alcohol 95% Histoprep
Fisher
HC13001GL
Histology
Tissue Path™ Disposable Embedding Rings
Fisher
22038198
Histology
Xylenes (Histological)
Fisher Chemical
X3P-1GAL
Histology
10x Tris/Glycine/SDS Buffer
Bio-Rad
1610732
Western Blot
2-Mercaptoethanol
Bio-Rad
1610710
Western Blot
4x Laemmli Sample Buffer
Bio-Rad
1610747
Western Blot
BCA Protein Assay Kit
Pierce
23225
Western Blot
Glycine
Sigma
G-7126
Western Blot
Methanol (100%)
Ficher
A412-4
Western Blot
Pierce Protease and Phosphatase Inhibitor Mini Tablets EDTA-Free
Thermo
A32961
Western Blot
Pierce RIPA Buffer
Thermo Scientific
89901
Western Blot
Precision Plus Protein Dual Color Standards
Bio-Rad
1610374
Western Blot
Restore Western Blot Stripping Buffer
Thermo
21059
Western Blot
SDS
Fisher
BP166-500
Western Blot
Spectra Multicolor High Range Protein Ladder
Thermo Scientific
26625
Western Blot
TRIzol Reagent
Ambion
15596018
Western Blot
Tris Base
Fisher
BP154-1
Western Blot
Tween-20
Santa Cruz
SC-29913
Western Blot
PowerUp SYBR Green Master Mix
Applied Biosystems
A25742
qPCR
RNaseZap
Sigma
R2020-250ML
qPCR
SuperScript IV VILO Master Mix
Invitrogen
11766050
qPCR
TRIzol™ Reagent
Invitrogen
15-596-018
qPCR
Sodium Chloride
Fisher
S630-10
–
Deposited Data
REAGENT or RESOURCE
SOURCE
IDENTIFIER
A molecular single-cell lung atlas of lethal COVID-19
GEO
GSE171524
–
Learning
Cells of the adult human heart
Human Cell Atlas
DOI: 10.1038/s41586-020-2797-4
–
Learning
Experimental models: Cell Lines
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Project
Purpose
Experimental models: Organisms and Strains
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Oligonucleotides
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Recominant DNA
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Software Algorithms
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Other
<>:7: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
<>:7: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
/tmp/ipykernel_2776/294112252.py:7: SyntaxWarning: "\." is an invalid escape sequence. Such sequences will not work in the future. Did you mean "\\."? A raw string is also an option.
other = other.astype(str).replace('\.0', '', regex=True)
REAGENT or RESOURCE
SOURCE
IDENTIFIER
Experiment
Category
BD Vacutainer Serum
BD
367815
Blood test
–
BD Vacutainer Sodium Herparin
BD
367878
Blood test
–
HbA1c Controls
Afinion
1115178
Blood test
–
HbA1c Test Kit
Afinion
1115015
Blood test
–
Nova Max Glucose Test Strips
Nova
43523
Blood test
–
Nova Max Plus
Nova
43436
Blood test
Hardware
Gentamicin (50 mg/mL)
Glibco
15750060
Cell culture
Drug
MicroAmp EnduraPlate Optical 384-well clear reaction plate with barcode